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Future Fields

Ento-Tf Transferrin

Regular price $36.50 USD
Regular price Sale price $36.50 USD
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Future Fields Ento-Tf is a Transferrin (Recombinant Bovine) Enriched Growth Media Supplement that comes in 1000X concentrate and is produced with the EntoEngine™ expression system.

Ento-Tf strongly binds to iron atoms and transports it into the cell, ensuring all the iron metabolism needs of the cells are met. Alongside excellent functionality, Ento-Tf is a cost effective alternative to other recombinant Transferrin products on the market.

Ento-Tf comes as a lyophilized powder, which when reconstituted is ready to be supplemented into cell culture media.

Future Fields products are currently available for Research Use Only.

Please see below for supporting documentation.

Please email sales@futurefields.io for bulk purchase discounts.


Synonym

Transferrin, Ento-Tf, TF, Tf

Description

Transferrin is a glycoprotein that promotes cell proliferation by tightly binding and delivering iron into the cells via the Transferrin-receptor. It is a bioactive protein intended for use in cell culture applications. Tf is an extracellular antioxidant, sequestering available iron and reducing the production of free radicals. Similarly, Tf has an antimicrobial function, impeding bacterial survival. Recombinant Bovine serotransferrin produced by the EntoEngineTM is a single glycosylated polypeptide chain containing 704 amino acids and has a molecular mass of 77 kDa.

Sequence (monomer):

MRPAVRALLACAVLGLCLADPERTVRWCTISTHEANKCASFRENVLRILESGPFVSCVKKTSHMDCIKAISNNEADAVTLDGGLVYEAGLKPNNLKPVVAEFHGTKDNPQTHYYAVAVVKKDTDFKLNELRGKKSCHTGLGRSAGWNIPMAKLYKELPDPQESIQRAAANFFSASCVPCADQSSFPKLCQLCAGKGTDKCACSNHEPYFGYSGAFKCLMEGAGDVAFVKHSTVFDNLPNPEDRKNYELLCGDNTRKSVDDYQECYLAMVPSHAVVARTVGGKEDVIWELLNHAQEHFGKDKPDNFQLFQSPHGKDLLFKDSADGFLKIPSKMDFELYLGYEYVTALQNLRESKPPDSSKDECMVKWCAIGHQERTKCDRWSGFSGGAIECETAENTEECIAKIMKGEADAMSLDGGYLYIAGKCGLVPVLAENYKTEGESCKNTPEKGYLAVAVVKTSDANINWNNLKDKKSCHTAVDRTAGWNIPMGLLYSKINNCKFDEFFSAGCAPGSPRNSSLCALCIGSEKGTGKECVPNSNERYYGYTGAFRCLVEKGDVAFVKDQTVIQNTDGNNNEAWAKNLKKENFEVLCKDGTRKPVTDAENCHLARGPNHAVVSRKDKATCVEKILNKQQDDFGKSVTDCTSNFCLFQSNSKDLLFRDDTKCLASIAKKTYDSYLGDDYVRAMTNLRQCSTSKLLEACTFHKP

Functional Equivalence

10 mL of Ento-Tf is approximately equivalent to the performance of 107 mg of recombinant bovine transferrin based on proliferation studies in C2C12 cells.

Source

Drosophila melanogaster


Documents
SDS
COA
Specsheet 

Articles
Future Fields' Transferrin Performance
September 28, 2021


Frequently Asked Questions

What is the shelf-life of this product?
The shelf-life is in our specification sheet.

The product arrived but was shipped at room temperature, is it OK?
Yes. We ship this product lyophilized. Please see the specification sheet for suggsted on-site storage conditions.

How do I get a Certificate of Analysis?
Please see above for an example CoA. If you require a lot-specific CoA, please email us at sales@futurefields.io.

Can this product be used to culture cells other than bovine species?
Yes! We have seen our products used successfully in a variety of cell cultures across land-dwelling and aquatic species.

What is the concentration of recombinant Albumin in this product?
All Ento- products are a very complex mixture of biomolecules which are enriched in the protein(s) of interest (recombinant bovine transferrin, in this case). Due to this complexity, the protein(s) of interest's actual concentration is exceedingly difficult to quantify. To suggest initial working quantities, we reference Ento- products with a metric we refer to as "functional equivalence" which is based on our in-house assays. Functional equivalence is the equivalent amount of isolated protein of interest that is needed to replicate the performance of this product.

This product is rigorously tested to ensure that both the background molecular profile of the supplement and the protein of interest contribute beneficial effects to cell growth and pass all safety testing requirements.

Has any safety testing been performed for this product?
Please see above for an example CoA which describes our safety testing results.

Are volume discounts available for this product?
Yes. Please reach out to sales@futurefields.io to discuss your needs.