High-purity recombinant bovine fibroblast growth factor 2. For research use only.
Future Fields
Recombinant Bovine FGF2
Regular price
$110.00 USD
Regular price
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$110.00 USD
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Product at a Glance
- Synonyms: Basic Fibroblast Growth Factor 2
- Uniprot: P03969
- MW: 17.3 kDa
- Purity: ≥ 95% by SDS-PAGE quantitative densitometry
- Expression Host: Drosophila melanogaster
- Lyophilized from: PBS, pH 7.4
- Endotoxin: < 1 EU/µg protein
- Mycoplasma by PCR: Negative
- For reconstitution and storage, refer to PDS
- Shipped as a lyophilized product, stable at room temperature for travel
Product Applications
- Stem cell culture: Supports the self-renewal and proliferation of ESCs and iPSCs in defined media by activating key signaling pathways such as PI3K/Akt and ERK1/2.
- Tissue engineering: Enhances cell proliferation, differentiation, and angiogenesis, aiding the repair and regeneration of cartilage, bone, muscle, and nerve tissue.
- Cellular agriculture: Promotes the growth of bovine satellite cells and myoblasts.
Product Sustainability
Manufactured in Future Fields' Green-certified lab located in Edmonton, Canada.
Quality Assays
Purity
Analysis method: SDS-PAGE under reducing conditions
Staining: SYPRO™ Ruby (Cat. # S12000)
Purity: 96% by quantitative densitometry
Molecular weight: Bovine FGF2 migrates at 16.5 kDa and 17.2 kDa
Molecular weight marker: Precision Plus Protein™ Unstained Standards (Cat. # 1610363)
SPR Functional Activity
Binding partner: Human FGF R2 (IIIb) protein, Fc Tag (FGF R2 Fc) (Cat. # FGB-H5256-100ug)
Capture method: Human FGF R2 Fc captured on a carboxymethyl dextran chip via Protein A surface
Affinity constant: 0.23 nM
Instrument type: Reichert2SPR
Cell Biological Activity
Cell line: NIH/3T3 (ATCC CRL-1658)
Assay: alamarBlue™ Cell Proliferation Assay
Activity: EC50 = 1.99 ng/mL
Additional Characterization
Identity verified by mass spectrometry and western blot. Results available on request.
Description
Fibroblast Growth Factor 2 (FGF2), also known as basic FGF (bFGF), is a critical member of the fibroblast growth factor family, which regulates essential biological processes including cell proliferation, differentiation, migration, and angiogenesis. FGF2 is pivotal in embryonic development and adult tissue maintenance, playing key roles in wound healing, angiogenesis, and stem cell proliferation.
FGF2 binds to and activates four transmembrane fibroblast growth factor receptors (FGFR1-4), triggering intracellular signaling pathways such as the RAS/MAPK, PI3K/Akt, and PLCγ. These pathways regulate diverse cellular functions, making FGF2 essential for research and biotechnology applications.
Recombinant bovine FGF2 is widely used in cell culture for maintaining and expanding pluripotent stem cells (ESCs and iPSCs), stimulating endothelial and mesenchymal stem cell growth, and promoting chondrogenic differentiation. This highly conserved protein shares 99% sequence homology with its human counterpart, ensuring robust functionality in cross-species applications.
Produced using advanced recombinant technology, our bovine FGF2 offers high purity, stability, and bioactivity, making it ideal for tissue engineering, regenerative medicine, and veterinary research. It is an indispensable component in defined media formulations and a valuable tool in bovine cellular agriculture and therapeutic development.
Publications
- 2023 A simple and robust serum-free media for the proliferation of muscle cells
(Food Research International)
https://doi.org/10.1016/j.foodres.2023.113194 - 2023 Satellite cells sourced from bull calves and dairy cows differs in proliferative and myogenic capacity – Implications for cultivated meat
(Food Research International)
https://doi.org/10.1016/j.foodres.2023.113217
FAQ
Additional Products
Recombinant Human FGF2
Recombinant Human PDGF-BB
Recombinant Human Prolactin, Fc Tag
