Recombinant Bovine FGF2

Keywords: Bovine satellite cells; serum-free media optimization; cultivated meat; image-based cytometry; transcriptomics

Researchers from Aarhus University, Denmark, in collaboration with Canadian universities, University of Calgary and University of Toronto, have recently revealed an advanced serum-free formulation designed for growing animal cells for consumption purposes. This optimized system incorporates key components like Future Fields' Recombinant Bovine FGF2 (2 ng/mL), fetuin (600 µg/mL), BSA (75 µg/mL), and insulin-transferrin-selenium (ITS) supplement, enabling robust proliferation of satellite cells from bovine, porcine, and mouse sources. Termed Tri-basal 2.0+, this cutting-edge composition was characterized using state-of-the-art technologies such as Design of Experiment (DOE), Response Surface Methodology (RSM), image-based cytometry, transcriptomics, and various live cell assays. This breakthrough holds immense promise for the cellular agriculture industry's efforts to produce large quantities of animal cells for consumption.

Keywords: Cellular agriculture; donor type; bovine satellite cell sourcing; cell proliferation; myogenic differentiation; label-free quantification; image-based cytometry; transcriptomics.

Aarhus University researchers conducted consecutive studies investigating cell types and growth media for cultivated meat production. They compared primary muscle satellite cells (SCs) from bull calves and dairy cows, observing distinct phenotypes and proliferation capacities. The research by Skirivergaard et al. revealed that although bull calf SCs exhibited better proliferation, their myotube formation resembled that of dairy SCs. Interestingly, the use of in-house serum-free media reduced biological variance among replicate animals within each donor type. These findings highlight the significance of both cell origin and consistent, functional optimized media formulations, as they can significantly impact final biomass and cultivated meat production.