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Future Fields
Recombinant Human Interleukin-10, Tag Free
Regular price
$303.85 USD
Regular price
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$303.85 USD
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Powered by people, fly by design. Future Fields’ Recombinant Human Interleukin-10 is a cytokine ready to support your life-changing research in anti-inflammatory research and beyond.
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a key anti-inflammatory cytokine that regulates immune responses. Encoded by the IL10 gene, it prevents inflammatory and autoimmune diseases by inhibiting pro-inflammatory cytokines like IFN-γ, TNF-α, and IL-1β through the JAK-STAT signaling pathway. IL-10 is produced by immune cells such as T helper cells, monocytes, and macrophages, as well as non-immune cells like epithelial cells. It downregulates Th1 cytokines and enhances B cell survival. Additionally, IL-10 can stimulate immune responses, such as enhancing CD8+ T cell activity in cancer immunotherapy. This dual role makes IL-10 a promising target for therapeutic applications in autoimmune disorders, cancer, and infections.
For Research Use Only.
Synonym
Interleukin-10, IL-10, cytokine synthesis inhibitory factor, CSIF, IL10, RP11-262N9.1, IL10A, MGC126450, MGC126451
Description
Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is a key anti-inflammatory cytokine that regulates immune responses. Encoded by the IL10 gene, it prevents inflammatory and autoimmune diseases by inhibiting pro-inflammatory cytokines like IFN-γ, TNF-α, and IL-1β through the JAK-STAT signaling pathway. IL-10 is produced by immune cells such as T helper cells, monocytes, and macrophages, as well as non-immune cells like epithelial cells. It downregulates Th1 cytokines and enhances B cell survival. Additionally, IL-10 can stimulate immune responses, such as enhancing CD8+ T cell activity in cancer immunotherapy. This dual role makes IL-10 a promising target for therapeutic applications in autoimmune disorders, cancer, and infections.
Sequence (monomer)
*GPGGSGGSGGS
Sequence: Full sequence available upon request.
Total: 171 aa
Theoretical pI: 7.82
Calculated MW: 19.4 kDa
Purity
≥ 90% pure, verified by SDS PAGE. Identity has been Verified by SDS PAGE anti-IL-10 antibody western blot. Confirmed during characterization by mass spec. Data is available upon request.
Species
Homo sapiens
Source
Drosophila melanogaster (Why flies?)
Product Data and Documents
Purity
Test Name: SDS-PAGE
Purity of recombinant human IL-10 was determined by SDS-PAGE. The protein was loaded at 100 ng and resolved in reducing conditions. The gel was stained using SYPRO™– a highly sensitive fluorescent dye.
Activity
Test Name: Surface Plasmon Resonance (SPR)
Activity of Human IL-10, Tag Free was determined by SPR using a receptor (Human IL-10 R alpha / CD210 Protein, Fc Tag (MALS verified) captured by Protein A immobilized on a carboxymethyl dextran chip.
Identity
Test Name: Western Blot
Identity of recombinant human IL-10 was confirmed by Western Blot. The protein was loaded at 30 ng and resolved in reducing conditions. The blot was developed using IL-10 mouse antibody.
Safety
Endotoxin: <1 EU/µg
Mycoplasma: Negative by qPCR
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Frequently Asked Questions
Why can’t I see a pellet in the vial?
Lyophilized powder may not be visible for many reasons including, but not limited to, dislodged powder being stuck on the cap or lack of carrier proteins that make the product difficult to see. Please tap or centrifuge the vial to bring all the material down to the bottom and reconstitute the product as outlined above.
Are there any stability concerns with freeze thawing?
Repeated freeze thawing is not recommended, as this may damage the protein products resulting in reduced functionality. After reconstitution, please aliquot into suitable sizes for one time use and freeze.
Can I add BSA as a carrier?
Yes. Adding a carrier protein like BSA (0.1%) enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.
Is the protein bioactive?
Yes, this protein was tested for activity on its cognate receptor via SPR.
Are volume discounts or recurring order discounts available for this product?
Yes. Please reach out to sales@futurefields.io to discuss your needs.
Sustainability
1% of your purchase proceeds will go towards environmental initiatives.
Scoring 86/100, we achieved the highest level of My Green Lab certification (Green).
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