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Future Fields
Recombinant Human Interleukin-2, Tag Free
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$125.48 USD
Regular price
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$125.48 USD
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Powered by people, fly by design. Future Fields’ Recombinant Human Interleukin-2 is a cytokine ready to support your life-changing research in cell therapies and beyond.
Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor. It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival.
Recombinant human IL-2 is produced to aid in various research and therapeutic applications, including CAR-T, CAR-NK, and TIL immunotherapies.
Our purified Recombinant Human Interleukin-2 comes as lyophilized powder, which when reconstituted is ready to be supplemented into cell culture media. Please see the Product Data Sheet for recommendations on storage, handling, and reconstitution.
For Research Use Only.
Synonym
T cell growth factor (TCGF)
Description
Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor. It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival. Recombinant human IL-2 is produced to aid in various research and therapeutic applications.
Sequence (monomer):
*Short 6 amino acid overhang post-cleavage.
Sequence: Full sequence available upon request.
Theoretical pI: 6.36
Calculated MW: 16.1 kDa
Purity
Verified by SDS PAGE to be >90% pure. Identity verified by Western blot. Data available upon request.
Species
Homo sapiens
Source
Drosophila melanogaster (Why flies?)
Product Data and Documents
Purity
Test Name: SDS-PAGE
Purity of recombinant human IL-2 was determined by SDS-PAGE. The protein was loaded at 100 ng and resolved in reducing conditions. The gel was stained using SYPRO™– a highly sensitive fluorescent dye.
Activity
Test Name: Surface Plasmon Resonance (SPR)
Activity of Human IL-2, Tag Free was determined by SPR using a receptor (Human IL-2RB&IL-2RA&IL-2RG, Fc Tag) captured by Protein A immobilized on a carboxymethyl dextran chip.
Identity
Test Name: Western Blot
Identity of recombinant human IL-2 was confirmed by Western Blot. The protein was loaded at 30 ng and resolved in reducing conditions. The blot was developed using IL-2 rabbit antibody.
Safety
Endotoxin: <1 EU/µg
Mycoplasma: Negative by qPCR
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Frequently Asked Questions
Why can’t I see a pellet in the vial?
Lyophilized powder may not be visible for many reasons including, but not limited to, dislodged powder being stuck on the cap or lack of carrier proteins that make the product difficult to see. Please tap or centrifuge the vial to bring all the material down to the bottom and reconstitute the product as outlined above.
Are there any stability concerns with freeze thawing?
Repeated freeze thawing is not recommended, as this may damage the protein products resulting in reduced functionality. After reconstitution, please aliquot into suitable sizes for one time use and freeze.
Can I add BSA as a carrier?
Yes. Adding a carrier protein like Bovine Serum Albumin (BSA 0.1%) enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.
Is the protein bioactive?
Yes, this protein was tested for activity on its cognate receptor via SPR.
Are volume discounts or recurring order discounts available for this product?
Yes. Please reach out to sales@futurefields.io to discuss your needs.
Sustainability
1% of your purchase proceeds will go towards environmental initiatives.
Scoring 86/100, we achieved the highest level of My Green Lab certification (Green).
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Future Fields Sustainability Committee works across departments to build our internal sustainability capacity. Read more about our commitment to sustainability here.
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