High-purity recombinant human Interleukin-2. For research use only.
Future Fields
Recombinant Human Interleukin-2
Regular price
$125.48 USD
Regular price
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$125.48 USD
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Product at a Glance
- Synonyms: IL-2, IL2, T cell Growth Factor (TCGF)
- Uniprot: Q0GK43
- MW: 16.1 kDa
- Purity: ≥ 90% by SDS-PAGE quantitative densitometry
- Expression Host: Drosophila melanogaster
- Lyophilized from: 50 mM Tris-HCl, 150 mM NaCl, pH 7.4
- Endotoxin: < 1 EU/µg protein
- Mycoplasma by PCR: Negative
- For reconstitution and storage, refer to PDS
- Shipped as a lyophilized product, stable at room temperature for travel
Product Applications
- Immune activation and proliferation: IL-2 plays a central role in activating T cells and natural killer (NK) cells, promoting their proliferation and enhancing their cytotoxic functions.
- T cell differentiation and expansion: IL-2 supports the differentiation of T cells into specialized subsets, including effector T cells, which are crucial for attacking pathogens and abnormal cells.
- Regulation of immune balance: By fostering the development and maintenance of regulatory T cells (Tregs), IL-2 helps suppress excessive immune responses and prevents autoimmunity.
Product Sustainability
Manufactured in Future Fields' Green-certified lab located in Edmonton, Canada.
Quality Assays
Purity
Analysis method: SDS-PAGE under reducing conditions
Staining: SYPRO™ Ruby (Cat. # S12000)
Purity: 100% by quantitative densitometry
Molecular weight: Human IL-2 migrates at 15.2 kDa
Molecular weight marker: Precision Plus Protein™ Unstained Standards (Cat. # 1610363)
SPR Functional Activity
Binding partner: Human IL-2 receptor (alpha, beta, and gamma), Fc Tag (IL-2 R Fc) (Cat. # ILG-H5257-100µg)
Capture method: Human IL-2 R Fc captured on a carboxymethyl dextran chip via Protein A surface
Affinity constant: 1.83 nM
Instrument type: Reichert2SPR
Cell Biological Activity
Cell line: CTLL-2 (ATCC TIB-214)
Assay: alamarBlue™ Cell Proliferation Assay
Activity: EC50 = 0.80 ng/mL
Additional Characterization
Identity verified by mass spectrometry and western blot. Results available on request.
Description
Interleukin-2 (IL-2), also known as T cell Growth Factor (TCGF), is a multifunctional cytokine critical for immune system regulation. IL-2 is primarily produced by activated CD4+ T cells and, to a lesser extent, by CD8+ T cells following antigen stimulation. It plays a central role in T cell proliferation, survival, and differentiation, particularly in the expansion of effector T cells and regulatory T cells (Tregs), thus maintaining immune homeostasis. IL-2 is a single-chain glycoprotein composed of 133 amino acids, with a molecular weight of 15.5 kDa. Its biological effects are mediated through its receptor complex, the IL-2 receptor (IL-2R), which consists of three subunits: IL-2Rα (CD25), IL-2Rβ (CD122), and the common gamma chain (γc, CD132). The receptor exists in three forms with varying affinities, and IL-2 binding activates downstream signaling pathways, including JAK-STAT, PI3K-Akt, and MAPK. The selective activation of these pathways depends on the receptor composition and cellular context.
IL-2 has broad therapeutic potential. It is an established agent in cancer immunotherapy, particularly for treating metastatic melanoma and renal cell carcinoma, where it boosts immune responses to attack tumors. Low-dose IL-2 is being investigated for autoimmune diseases such as lupus and type 1 diabetes, as it selectively promotes Treg expansion to restore immune balance. Additionally, IL-2 has shown promise in chronic infections like HIV and hepatitis C by enhancing immune cell recovery and function.
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