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Future Fields
Recombinant Human PDGF-BB, Tag Free
Regular price
$292.53 USD
Regular price
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$292.53 USD
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Product at a Glance
High-purity platelet-derived growth factor BB (PDGF-BB) with no tag. For research use only.
- Uniprot: P01127
- MW: 13.6 kDa monomer.
- Purity: ≥ 95% by SDS PAGE quantitative densitometry.
- Expression Host: Drosophila melanogaster
- Lyophilized from: 50 mM Sodium Acetate pH 5, 100 mM NaCl.
- Endotoxin: <1 EU/µg protein.
- Mycoplasma by PCR: Negative.
- For reconstitution and storage refer to PDS.
- Shipped as a lyophilized product, stable at room temperature for travel.
Product Applications
- Cellular growth and signaling pathways: PDGF-BB can be used to explore the regulatory mechanisms of cell proliferation, differentiation, and survival, especially for fibroblasts and smooth muscle cells.
- Angiogenesis and vascular biology: Widely used in research to study vascular development, remodeling, and its role in diseases such as atherosclerosis and cancer. PDGF-BB plays a vital role in angiogenesis and vascular biology by promoting blood vessel formation, enhancing endothelial cell function, and stabilizing vascular structures.
- Tissue repair and regeneration: PDGF-BB is studied for its ability to enhance wound healing, bone regeneration, and connective tissue repair, with its potential applications evaluated in tissue engineering and regenerative medicine research.
Product Sustainability
Manufactured in Future Fields' Green-certified lab located in Edmonton, Canada.
Quality Assays
Purity
Analysis method: SDS-PAGE under reducing and non-reducing conditions.
Staining: SYPRO™ Ruby (Cat. # S12000).
Purity: 97% by quantitative densitometry.
Molecular weight: The monomer (reduced) migrates at 13.0 kDa. The dimer (non-reduced) migrates at 28.1 kDa.
Molecular weight marker: Precision Plus Protein™ Unstained Standard (Cat. # 1610363).
SPR Functional Activity
Binding partner: Human PDGF receptor beta (PDGFR-β), Fc tag (Cat. # PDB-H5259-100ug).
Capture method: Human PDGFR-β, Fc tag captured on a carboxymethyl dextran chip via Anti-human IgG Fc antibodies surface.
Affinity constant: 0.15 nM
Instrument type: Reichert2SPR.
Cell Biological Activity
Cell line: NIH/3T3 ATCC (CRL-1658™).
Assay: alamarBlue™ Cell Proliferation Assay.
Activity: EC50 = 14.1 ng/mL
Additional Characterization
Identity verified by mass spectrometry and western blot. Results available on request.
Description
Platelet-Derived Growth Factor-BB (PDGF-BB) is a key growth factor involved in wound healing, development, and tissue regeneration. A member of the PDGF family, PDGF-BB is a homodimer of two PDGF-B chains linked by disulfide bonds, with a molecular weight of 24.5 kDa and a conserved cystine knot motif crucial for stability and function. PDGF-BB’s structure allows it to effectively bind to its receptors, PDGFR-α and PDGFR-β, which are tyrosine kinase receptors located on the cell surface. Upon binding to these receptors, PDGF-BB induces receptor dimerization and autophosphorylation, triggering downstream signaling pathways such as PI3K/Akt, MAPK, and PLCγ driving cellular proliferation, migration, survival, and differentiation.
PDGF-BB plays a critical role in stimulating fibroblast and smooth muscle cell proliferation, promoting granulation tissue formation, and remodeling the extracellular matrix. It also enhances angiogenesis by recruiting pericytes and stabilizing blood vessels. Recombinant PDGF-BB is a powerful research tool for studying these processes and developing innovative approaches in vascular biology, tissue engineering, and regenerative medicine.
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