High-purity recombinant human prolactin. Tag located at the C-terminus of the protein. For research use only.
Future Fields
Recombinant Human Prolactin, Fc Tag
Regular price
$105.26 USD
Regular price
Sale price
$105.26 USD
Unit price
per
Couldn't load pickup availability
Request a sample or bulk order quote:
Product at a Glance
- Uniprot: P01236
- MW: 51.7 kDa
- Purity: ≥ 90% by SDS-PAGE quantitative densitometry
- Expression Host: Drosophila melanogaster
- Lyophilized from: 50 mM Tris-HCl, 100 mM NaCl, pH 7.4
- Endotoxin: < 1 EU/µg protein
- Mycoplasma by PCR: Negative
- For reconstitution and storage, refer to PDS
- Shipped as a lyophilized product, stable at room temperature for travel
Product Applications
- Research and development: The Fc tag enhances the stability and half-life of the protein, making it more suitable for long-term studies.
- Bioactivity and binding studies: The protein can be used to study the interaction between prolactin and its receptor (PRLR) or other binding partners. The Fc tag facilitates binding assays, such as ELISA, SPR, and BLI.
- Diagnostic applications: Fc-tagged prolactin can be used in diagnostic assays, such as ELISA or SPR (surface plasmon resonance), to measure prolactin levels or study its binding affinity to antibodies and other binding partners.
Product Sustainability
Manufactured in Future Fields' Green-certified lab located in Edmonton, Canada.
Quality Assays
Purity
Analysis method: SDS-PAGE under reducing conditions
Staining: SYPRO™ Ruby (Cat. # S12000)
Purity: 98% by quantitative densitometry
Molecular weight: Human PRL-Fc migrates at 51.2 kDa
Molecular weight marker: Precision Plus Protein™ Unstained Standards (Cat. # 1610363)
SPR Functional Activity
Binding partner: Human prolactin receptor (PRLR) (Cat. # PRR-H52Ha-100ug)
Capture method: Human PRLR captured on a carboxymethyl dextran chip via amine coupling
Affinity constant: 28.9 nM
Instrument type: Reichert2SPR
Cell Biological Activity
Cell line: Nb2-11 ECACC (Cat. # 97041101)
Assay: alamarBlue™ Cell Proliferation Assay
Activity: EC50 = 8.0 ng/mL
Additional Characterization
Identity verified by mass spectrometry and western blot. Results available on request.
Description
Prolactin is a multifunctional hormone primarily known for its role in lactation and regulating immune responses, reproduction, and cellular growth. It interacts with the prolactin receptor, a member of the cytokine receptor superfamily, to activate signaling pathways essential for these physiological processes.
Prolactin engineered with a C-terminal Fc tag, derived from immunoglobulins, offers enhanced utility for research and therapeutic development. The Fc tag improves protein stability and prolongs circulating half-life in vivo, making Fc-tagged prolactin an essential tool for receptor binding studies, therapeutic use, and diagnostic applications.
By combining the bioactivity of prolactin with the functional benefits of the Fc tag, this product offers a versatile solution for both fundamental research and translational applications.
Additional Products
Recombinant Human Prolactin
Recombinant Human Interleukin-2
Recombinant Human PDGF-BB
