Future Fields

Recombinant Human Prolactin

Name: Recombinant Human Prolactin
Brand: Future Fields
SKU: PRL-111-50µg
Price: 126.32 USD
Availability: InStock

$126.32 USD

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Product at a Glance

High-purity recombinant human prolactin. For research use only.

Uniprot: P01236
MW: 26.8 kDa
Purity: ≥ 90% by SDS-PAGE quantitative densitometry
Expression Host: Drosophila melanogaster
Lyophilized from: 50 mM Tris-HCl, 100 mM NaCl, pH 7.4
Endotoxin: < 1 EU/µg protein
Mycoplasma by PCR: Negative
For reconstitution and storage, refer to PDS
Shipped as a lyophilized product, stable at room temperature for travel

Product Applications

Hormone function studies: Investigate the physiological roles of prolactin in lactation, reproduction, and metabolism, as well as its impact on hormonal feedback mechanisms and endocrine system interactions.
Signal transduction and molecular pathways: Explore prolactin-mediated activation of JAK/STAT, MAPK, and PI3K/Akt pathways and identify downstream effects on cell proliferation, differentiation, and survival.
Immunological research: Examine immunomodulatory effects of prolactin on T cells, B cells, and macrophages, as well as its role in autoimmune diseases and inflammatory processes.

Product Sustainability

Manufactured in Future Fields' Green-certified Lab located in Edmonton, Canada.

Product Documents

Download SDS

Download PDS

Quality Assays

Purity

Analysis method: SDS-PAGE under reducing conditions
Staining: SYPRO™ Ruby (Cat. # S12000)
Purity: 94% by quantitative densitometry
Molecular weight: Human PRL migrates at 24.5 kDa and 26.2 kDa (glycosylated)
Molecular weight marker: Precision Plus Protein™ Unstained Standards (Cat. # 1610363)

SPR Functional Activity

Binding partner: Human prolactin receptor (PRLR) (Cat. # PRR-H52Ha-100ug)
Capture method: Human PRLR captured on a carboxymethyl dextran chip via amine coupling
Affinity constant: 23.7 nM
Instrument type: Reichert2SPR

Cell Biological Activity

Cell line: Nb2-11 ECACC (Cat. # 97041101)
Assay: alamarBlue™ Cell Proliferation Assay
Activity: EC50 = 0.3 ng/mL

Additional Characterization

Identity verified by mass spectrometry and western blot. Results available on request.

Relevant Resources

Insights into Glycosylation and Activity

Why Prolactin is Future Fields' Labour of Love

First-ever Fruit Fly-Derived Recombinant Human Prolactin

Description

Prolactin is a multifunctional hormone involved in critical biological processes such as lactation, immune modulation, reproduction, and cellular growth. Its activity is mediated through binding to the prolactin receptor (PRLR), a member of the cytokine receptor superfamily, leading to activation of several intracellular signaling pathways.

Upon receptor binding, prolactin primarily triggers the JAK-STAT (Janus kinase-signal transducer and activator of transcription) pathway, a key mediator of gene transcription for cell proliferation and differentiation. It also activates other pathways, including:

PI3K/Akt pathway: Regulates cell survival, growth, and metabolism
MAPK/ERK pathway: Promotes cellular proliferation and differentiation
Src Kinase pathway: Involved in cytoskeletal rearrangement and cell adhesion
NF-κB pathway: Plays a role in immune response and inflammation

This complex signaling network underpins the diverse physiological roles of prolactin, making prolactin a valuable tool for studying its native activity and functional interactions in both basic research and therapeutic exploration.