Future Fields

Recombinant Human Prolactin, Tag-Free

Name: Recombinant Human Prolactin, Tag-Free
Brand: Future Fields
SKU: PRL-111-50µg
Price: 126.32 USD
Availability: InStock

$126.32 USD

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Product at a Glance

High-purity recombinant tag-free human prolactin. For research use only.

Uniprot: P01236
MW: 26.8 kDa
Purity: ≥ 85% by SDS PAGE quantitative densitometry
Expression Host: Drosophila melanogaster
Lyophilized from: 50 mM Tris, 100 mM NaCl, pH 7.4
Endotoxin: <1 EU/µg protein
Mycoplasma by PCR: negative
For reconstitution and storage refer to PDS
Shipped at a lyophilized state, stable at room temperature for travel

Product Applications

Hormone function studies: Investigate the physiological roles of prolactin in lactation, reproduction, and metabolism, as well as its impact on hormonal feedback mechanisms and endocrine system interactions.
Signal transduction and molecular pathways: Explore prolactin-mediated activation of JAK/STAT, MAPK, and PI3K/Akt pathways and identify downstream effects on cell proliferation, differentiation, and survival.
Immunological research: Examine immunomodulatory effects of prolactin on T cells, B cells, and macrophages, as well as its role in autoimmune diseases and inflammatory processes.

Product Sustainability

Manufactured in Future Fields' Green-certified lab located in Edmonton, Canada.

Product Documents

Download SDS

Download PDS

Quality Assays

Purity

Analysis method: SDS-PAGE under reducing conditions
Staining: SYPRO™ Ruby
Purity: 94% by quantitative densitometry under reducing conditions
Molecular weight: hPRL migrates between 24.3 kDa and 26.1 kDa (glycosylated)
Molecular weight marker: Precision Plus Protein™ Unstained Standard (Cat. # 1610363)

SPR Functional Activity

Binding partner: Human prolactin receptor (PRLR) (Cat. # PRR-H52Ha-100ug)
Capture method: Human PRLR captured on a carboxymethyl dextran chip via amine coupling
Affinity constant: 23.7 nM
Instrument type: Reichert2SPR

Cell Biological Activity

Cell line: Nb2-11 ECACC (Cat. # 97041101)
Assay: alamarBlue™ Cell Proliferation Assay
Activity: EC50 = 0.33 ng/mL

Additional Characterization

Identity verified by mass spectrometry and western blot. Results available on request.

Relevant Resources

Insights into Glycosylation and Activity

Why Prolactin is Future Fields' Labour of Love

First-ever Fruit Fly-Derived Recombinant Human Prolactin

Description

Prolactin is a multifunctional hormone involved in critical biological processes such as lactation, immune modulation, reproduction, and cellular growth. Its activity is mediated through binding to the prolactin receptor (PRLR), a member of the cytokine receptor superfamily, leading to activation of several intracellular signaling pathways.

Upon receptor binding, prolactin primarily triggers the JAK-STAT (Janus kinase-signal transducer and activator of transcription) pathway, a key mediator of gene transcription for cell proliferation and differentiation. It also activates other pathways, including:

PI3K-AKT Pathway: Regulates cell survival, growth, and metabolism.
MAPK/ERK Pathway: Promotes cellular proliferation and differentiation.
Src Kinase Pathway: Involved in cytoskeletal rearrangement and cell adhesion.
NF-κB Pathway: Plays a role in immune response and inflammation.

This complex signaling network underpins prolactin’s diverse physiological roles, making tag-free prolactin a valuable tool for studying its native activity and functional interactions in both basic research and therapeutic exploration.