High-purity recombinant human prolactin. For research use only.
Recombinant Human Prolactin
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Product at a Glance
- Uniprot: P01236
- MW: 26.8 kDa
- Purity: ≥ 90% by SDS-PAGE quantitative densitometry
- Expression Host: Drosophila melanogaster
- Lyophilized from: 50 mM Tris-HCl, 100 mM NaCl, pH 7.4
- Endotoxin: < 1 EU/µg protein
- Mycoplasma by PCR: Negative
- For reconstitution and storage, refer to PDS
- Shipped as a lyophilized product, stable at room temperature for travel
Product Applications
- Hormone function studies: Investigate the physiological roles of prolactin in lactation, reproduction, and metabolism, as well as its impact on hormonal feedback mechanisms and endocrine system interactions.
- Signal transduction and molecular pathways: Explore prolactin-mediated activation of JAK/STAT, MAPK, and PI3K/Akt pathways and identify downstream effects on cell proliferation, differentiation, and survival.
- Immunological research: Examine immunomodulatory effects of prolactin on T cells, B cells, and macrophages, as well as its role in autoimmune diseases and inflammatory processes.
Product Sustainability
Manufactured in Future Fields' Green-certified Lab located in Edmonton, Canada.
Quality Assays
Purity
Analysis method: SDS-PAGE under reducing conditions
Staining: SYPRO™ Ruby (Cat. # S12000)
Purity: 94% by quantitative densitometry
Molecular weight: Human PRL migrates at 24.5 kDa and 26.2 kDa (glycosylated)
Molecular weight marker: Precision Plus Protein™ Unstained Standards (Cat. # 1610363)
SPR Functional Activity
Binding partner: Human prolactin receptor (PRLR) (Cat. # PRR-H52Ha-100ug)
Capture method: Human PRLR captured on a carboxymethyl dextran chip via amine coupling
Affinity constant: 23.7 nM
Instrument type: Reichert2SPR
Cell Biological Activity
Cell line: Nb2-11 ECACC (Cat. # 97041101)
Assay: alamarBlue™ Cell Proliferation Assay
Activity: EC50 = 0.3 ng/mL
Additional Characterization
Identity verified by mass spectrometry and western blot. Results available on request.
Description
Prolactin is a multifunctional hormone involved in critical biological processes such as lactation, immune modulation, reproduction, and cellular growth. Its activity is mediated through binding to the prolactin receptor (PRLR), a member of the cytokine receptor superfamily, leading to activation of several intracellular signaling pathways.
Upon receptor binding, prolactin primarily triggers the JAK-STAT (Janus kinase-signal transducer and activator of transcription) pathway, a key mediator of gene transcription for cell proliferation and differentiation. It also activates other pathways, including:
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PI3K/Akt pathway: Regulates cell survival, growth, and metabolism
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MAPK/ERK pathway: Promotes cellular proliferation and differentiation
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Src Kinase pathway: Involved in cytoskeletal rearrangement and cell adhesion
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NF-κB pathway: Plays a role in immune response and inflammation
This complex signaling network underpins the diverse physiological roles of prolactin, making prolactin a valuable tool for studying its native activity and functional interactions in both basic research and therapeutic exploration.